Krühne, Ulrich1; Heintz, Søren3; Ringborg, Rolf Hoffmeyer1; Pereira Rosinha, Ines1; Tufvesson, Pär1; Gernaey, Krist V.1; Woodley, John M.1
1 Department of Chemical and Biochemical Engineering, Technical University of Denmark2 Center for Process Engineering and Technology, Department of Chemical and Biochemical Engineering, Technical University of Denmark3 Department of Chemistry, Technical University of Denmark
The increasing interest in biocatalytic processes means there is a clear need for a new systematic development paradigm which encompasses both protein engineering and process engineering. This paper argues that through the use of a new microfluidic platform, data can be collected more rapidly and integrated with process modeling, can provide the basis for validating a reduced number of potential processes. The miniaturized platform should use a smaller reagent inventory and make better use of precious biocatalysts. The EC funded BIOINTENSE project will use ω-transaminase based synthesis of chiral amines as a test-bed for assessing the viability of such a high throughput biocatalytic process development, and in this paper, such a vision for the future is presented.
Green Processing and Synthesis, 2014, Vol 3, Issue 1, p. 23-31
Biocatalysis; Microfluidics; Process development; Transaminase