Choi, Moon-Hee7; Hwang, Yuhoon1; Uk Lee, Hyun8; Kim, Bohwa9; Lee, Go-Woon9; Andersen, Henrik Rasmus1; Lee, Young-Chul10; Suk Huh, Yun10
1 Department of Environmental Engineering, Technical University of Denmark2 Urban Water Engineering, Department of Environmental Engineering, Technical University of Denmark3 Chosun University4 Korea Basic Science Institute5 Korea Institute of Energy Research6 Inha University7 Chosun University8 Korea Basic Science Institute9 Korea Institute of Energy Research10 Inha University
In the present study the short term aquatic ecotoxicity of water-solubilized aminoclay nanoparticles (ANPs) of ~51±31 nm average hydrodynamic diameter was characterized. An ecotoxicological evaluation was carried out utilizing standard test organisms of different phyla and trophic levels namely the eukaryotic microalga Pseudokirchneriella subcapitata, the crustacean Daphnia magna and the bioluminescent marine bacteria Vibrio fisheri. The effective inhibitory concentration (EC50) with 95% confidence limits for the microalga was 1.29 mg/L (0.72–1.82) for the average growth rate and 0.26 mg/L (0.23–0.31) for the cell yield. The entrapping of algal cells in aggregates of ANP may play a major role in the growth inhibition of algae P. subcapitata. No inhibition was observed for V. fisheri up to 25,000 mg/L (no observed effect concentration; NOEC). For D. magna no immobilization was observed in a limit test with 100 mg/L in 24 h while in 48 h a single animal was immobilized (5% inhibition). Correspondingly, the NOEC of ANP in 24 h was 100 mg/L and the lowest observed effect concentration (LOEC) for 48 h was 100 mg/L. Therefore it can be considered to use ANP as an algal-inhibition agent at concentrations <100 mg/L without affecting or only mildly affecting other organisms including zooplanktons, but further studies on the environmental fate and chronic toxicity of ANP is needed to confirm this.
Ecotoxicology and Environmental Safety, 2014, Vol 102, Issue 1, p. 34-41