Hansen, Mette Reimert1; Le Quement, Sebastian Thordal1; Jakobsen, Tim Holm4; Skovstrup, Søren5; Taboureau, Olivier6; Tolker‐Nielsen, Tim7; Givskov, Michael Christian7; Nielsen, Thomas Eiland8
1 Department of Chemistry, Technical University of Denmark2 Organic Chemistry, Department of Chemistry, Technical University of Denmark3 Center for Biological Sequence Analysis, Department of Systems Biology, Technical University of Denmark4 Department of Systems Biology, Technical University of Denmark5 Technical University of Denmark6 Department of Bio and Health Informatics, Technical University of Denmark7 University of Copenhagen8 Department of Organic Chemistry, Technical University of Denmark
Bacteria use small signaling molecules to communicate in a process termed “quorum sensing” (QS), which enables the coordination of survival strategies, such as production of virulence factors and biofilm formation. In Gram‐negative bacteria, these signaling molecules are a series of N‐acylated L‐homoserine lactones. With the goal of identifying non‐native compounds capable of modulating bacterial QS, a virtual library of N‐dipeptido L‐homoserine lactones was screened in silico with two different crystal structures of LasR. The 30 most promising hits were synthesized on HMBA‐functionalized PEGA resin and released through an efficient acid‐mediated cyclative release mechanism. Subsequent screening for modulation of QS in Pseudomonas aeruginosa and E. coli identified six moderately strong activators. A follow‐up library designed from the preliminary derived structure–activity relationships was synthesized and evaluated for their ability to activate the QS system in this bacterium. This resulted in the identification of another six QS activators (two with low micromolar activity) thus illuminating structural features required for QS modulation.