Roager, Henrik Munch1; Licht, Tine Rask3; Poulsen, Sanne4; Larsen, Thomas Meinert4; Bahl, Martin Iain1
1 National Food Institute, Technical University of Denmark2 Research Group for Gut Microbiology and Immunology, National Food Institute, Technical University of Denmark3 Copenhagen Center for Health Technology, Center, Technical University of Denmark4 University of Copenhagen
It has been suggested that the human gut microbiota can be divided into enterotypes based on the abundance of specific bacterial groups; however, the biological significance and stability of these enterotypes remain unresolved. Here, we demonstrated that subjects (n = 62) 18 to 65 years old with central obesity and components of metabolic syndrome could be grouped into two discrete groups simply by their relative abundance of Prevotella spp. divided by Bacteroides spp. (P/B ratio) obtained by quantitative PCR analysis. Furthermore, we showed that these groups remained stable during a 6-month, controlled dietary intervention, where the effect of consuming a diet in accord with the new Nordic diet (NND) recommendations as opposed to consuming the average Danish diet (ADD) on the gut microbiota was investigated. In this study, subjects (with and without stratification according to P/B ratio) did not reveal significant changes in 35 selected bacterial taxa quantified by quantitative PCR (ADD compared to NND) resulting from the dietary interventions. However, we found higher total plasma cholesterol within the high-P/B group than in the low-P/B group after the intervention. We propose that stratification of humans based simply on their P/B ratio could allow better assessment of possible effects of interventions on the gut microbiota and physiological biomarkers.
Applied and Environmental Microbiology, 2014, Vol 80, Issue 3, p. 1142-1149