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1 Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark 2 Department of Environmental Engineering, Technical University of Denmark 3 Residual Resource Engineering, Department of Environmental Engineering, Technical University of Denmark 4 Water Resources Engineering, Department of Environmental Engineering, Technical University of Denmark 5 Department of Systems Biology, Technical University of Denmark 6 Capital Region of Denmark 7 Geological Survey of Denmark and Greenland 8 SiREM 9 Geological Survey of Denmark and Greenland
A molecular study on how the abundance of the dechlorinating culture KB-1 affects dechlorination rates in clay till is presented. DNA extracts showed changes in abundance of specific dechlorinators as well as their functional genes. Independently of the KB-1 added, the microbial dechlorinator abundance increased to the same level in all treatments. In the non-bioaugmented microcosms the reductive dehalogenase gene bvcA increased in abundance, but when KB-1 was added the related vcrA gene increased while bvcA genes did not increase. Modeling showed higher vinyl-chloride dechlorination rates and shorter time for complete dechlorination to ethene with higher initial concentration of KB-1 culture, while cis-dichloroethene dechlorination rates were not affected by KB-1 concentrations. This study provides high resolution abundance profiles of Dehalococcoides spp. (DHC) and functional genes, highlights the ecological behavior of KB-1 in clay till, and reinforces the importance of using multiple functional genes as biomarkers for reductive dechlorination. © 2013 Elsevier Ltd. All rights reserved.
Environmental Pollution, 2014, Vol 186, p. 149-157
Aluminum Silicates; Biodegradation, Environmental; DNA, Bacterial; Ethylenes; Halogenation; Kinetics; Models, Chemical; Soil Microbiology; Soil Pollutants; Vinyl Chloride; Chlorine compounds; Ethylene; Genes; Models; Polymerase chain reaction; Dechlorination
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