1 Department of Micro- and Nanotechnology, Technical University of Denmark2 Bioanalytics, Department of Micro- and Nanotechnology, Technical University of Denmark3 Colloids and Biological Interfaces, Department of Micro- and Nanotechnology, Technical University of Denmark4 Center for Intelligent Drug Delivery and Sensing Using Microcontainers and Nanomechanics, Center, Technical University of Denmark5 Politecnico di Milano6 Politecnico di Milano
A theoretical and experimental comparison between vertical and coplanar interdigitated sensing configurations for impedimetric cell growth tracking is presented. For the first time, these widely-adopted approaches are quantitatively compared on the same cell populations and on the same 10μm interdigitated microelectrodes using a versatile custom-made monitoring platform including a 24-channel miniaturized potentiostat. As expected, characterization of bare microelectrodes in buffer and tracking experiments with HeLa cells over 16 hours demonstrate that the coplanar configuration provides a higher sensitivity to cell adhesion and spreading (Cell Index = 1.6 vs. 0.4) albeit at a higher frequency of maximum sensitivity (100kHz vs. 24 kHz).
Proceedings of the International Workshop on Impedance Spectroscopy, 2012