Rizzi, Giovanni1; Østerberg, Frederik Westergaard1; Dufva, Martin5; Hansen, Mikkel Fougt1
1 Department of Micro- and Nanotechnology, Technical University of Denmark2 Magnetic Systems, Department of Micro- and Nanotechnology, Technical University of Denmark3 Department of Physics, Technical University of Denmark4 Fluidic Array Systems and Technology, Department of Micro- and Nanotechnology, Technical University of Denmark5 Center for Intelligent Drug Delivery and Sensing Using Microcontainers and Nanomechanics, Center, Technical University of Denmark
We present a magnetoresistive sensor platform for hybridization assays and demonstrate its applicability on single nucleotide polymorphism (SNP) genotyping. The sensor relies on anisotropic magnetoresistance in a new geometry with a local negative reference and uses the magnetic field from the sensor bias current to magnetize magnetic beads in the vicinity of the sensor. The method allows for real-time measurements of the specific bead binding to the sensor surface during DNA hybridization and washing. Compared to other magnetic biosensing platforms, our approach eliminates the need for external electromagnets and thus allows for miniaturization of the sensor platform.
Proceedings of the 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 2013, p. 1523-1525
Biosensor; SNP genotyping; Magnetoresistance; Lab-on-a-chip; DNA sensor
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17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 2013