Ísberg, Vignir6; Vroling, Bas4; van der Kant, Rob5; Li, Kang7; Vriend, Gert5; Gloriam, David8
1 Biostructural Research, Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, Københavns Universitet2 Department of Mathematical Sciences, Faculty of Science, Københavns Universitet3 Drug Research Academy M, Drug Research Academy, Faculty of Pharmaceutical Sciences, Københavns Universitet4 Bio-Prodict B.V., Nijmegen5 Radboudumc Nijmegen Medical Centre, Nijmegen6 Drug Research Academy M, Drug Research Academy, Faculty of Pharmaceutical Sciences, Københavns Universitet7 Department of Mathematical Sciences, Faculty of Science, Københavns Universitet8 Biostructural Research, Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, Københavns Universitet
an information system for G protein-coupled receptors
For the past 20 years, the GPCRDB (G protein-coupled receptors database; http://www.gpcr.org/7tm/) has been a 'one-stop shop' for G protein-coupled receptor (GPCR)-related data. The GPCRDB contains experimental data on sequences, ligand-binding constants, mutations and oligomers, as well as many different types of computationally derived data, such as multiple sequence alignments and homology models. The GPCRDB also provides visualization and analysis tools, plus a number of query systems. In the latest GPCRDB release, all multiple sequence alignments, and >65,000 homology models, have been significantly improved, thanks to a recent flurry of GPCR X-ray structure data. Tools were introduced to browse X-ray structures, compare binding sites, profile similar receptors and generate amino acid conservation statistics. Snake plots and helix box diagrams can now be custom coloured (e.g. by chemical properties or mutation data) and saved as figures. A series of sequence alignment visualization tools has been added, and sequence alignments can now be created for subsets of sequences and sequence positions, and alignment statistics can be produced for any of these subsets.