Proteomic Comparison between Maturation Drying and Prematurely Imposed Drying of <em>Zea mays</em> Seeds Reveals a Potential Role of Maturation Drying in Preparing Proteins for Seed Germination, Seedling Vigor, and Pathogen Resistance
Wang, Wei-Qing2; Ye, Jian-Qing2; Rogowska-Wrzesinska, Adelina3; Wojdyla, Katarzyna Iwona4; Jensen, Ole Nørregaard5; Møller, Ian Max6; Song, Song-Quan2
1 Department of Molecular Biology and Genetics - Afgrødegenetik og Bioteknologi, Department of Molecular Biology and Genetics, Science and Technology, Aarhus University2 Key Laboratory of Plant Resources, Institute of Botany, The Chinese Academy of Sciences3 Department of Biochemistry and Molecular Biology, University of Southern Danmark4 Department of Biochemistry and Molecular Biology, University of Southern Denmark5 Dept. of Biochemistry and Molecular Biology, University of Southern Denmark, Odense M6 Department of Molecular Biology and Genetics - Afgrødegenetik og Bioteknologi, Department of Molecular Biology and Genetics, Science and Technology, Aarhus University
We have studied the role(s) of maturation drying in the acquisition of germinability, seedling vigor and pathogen resistance by comparing the proteome changes in maize embryo and endosperm during mature and prematurely imposed drying. Prematurely imposed dried seeds at 40 days after pollination (DAP) germinated almost as well as mature seeds (at 65 DAP), but their seedling growth was slower and they were seriously infected by fungi. A total of 80 and 114 proteins were identified to change at least two-fold (p < 0.05) in abundance during maturation drying in embryo and endosperm, respectively. Fewer proteins (48 and 59 in embryo and endosperm, respectively) changed in abundance during prematurely imposed drying. A number of proteins, 33 and 38 in embryo and endosperm, respectively, changed similarly in abundance during both maturation and prematurely imposed drying. Storage proteins were abundant in this group and may contribute to the acquisition of seed germinability. However, a relatively large number of proteins changed in the embryo (47 spots) and endosperm (76 spots) specifically during maturation drying. Among these proteins, storage proteins in the embryo and defense proteins in the endosperm may be particularly important for seedling vigor and resistance to fungal infection, respectively.
Journal of Proteome Research, 2014, Vol 13, Issue 2, p. 606-626