Tan, Hsih-Yin1; Loke, Weng Keong3; Nguyen, Nam-Trung4; Tan, Swee Ngin5; Tay, Nam Beng7; Wang, Wei7; Ng, Sum Huan7
1 Department of Micro- and Nanotechnology, Technical University of Denmark2 Colloids and Biological Interfaces, Department of Micro- and Nanotechnology, Technical University of Denmark3 DSO National Laboratories4 Griffith University5 National Institute of Education6 Singapore Institute of Manufacturing Technology7 Singapore Institute of Manufacturing Technology
This paper reports a lab-on-a-chip for the detection of Sarin nerve agent based on rapid electrochemical detection. The chemical warfare agent Sarin (C4H10FO2P, O-isopropyl methylphosphonofluoridate) is a highly toxic organophosphate that induces rapid respiratory depression, seizures and death within minutes of inhalation. As purified Sarin is colourless, odourless, water soluble and a easily disseminated nerve agent, it has been used as a weapon in terrorist or military attacks. To ascertain whether potable water supplies have been adulterated with this extremely potent poison, an inexpensive, sensitive and easy to use portable test kit would be of interest to first responders investigating such attacks. We report here an amperometric-based approach for detecting trace amounts of Sarin in water samples using a screen-printed electrode (SPE) integrated in a microfluidic chip. Enzymatic inhibition was obtained by exposing the immobilised biosensor in the microfluidic platform to Sarin in water samples. With the aid of cobalt phthalocyanine modified SPE, the device could detect Sarin at part-per-billion levels with concentration as low as 1 nM. The detection method reported here represents a significant improvement over the authors’previous optical-based detection method.
Biomedical Microdevices, 2014, Vol 16, Issue 2, p. 269-275