1 The Department of Environmental, Social and Spatial Change, Roskilde University2 Environment, Energy, Transport - Regulation, Innovation, and Climate Policy, Department of People and Technology, Roskilde University3 Environmental Risk, Administration Department of Roskilde University, Roskilde University4 Environmental Dynamics, Department of Science and Environment, Roskilde University5 unknown
The aim of this study was to optimize the epithelioma papillosum cyprini (EPC) cell line handling procedure for the comet assay to investigate the genotoxic potential of widely used pesticides. The effects of various media and handling of the EPC cell line were examined. Results indicated that avoiding trypsin to detach cells led to lower level of DNA damage in the negative control. Further, two commonly used herbicides (Dezormon and Optica trio) and their four active ingredients (4-chloro-o-tolyloxyacetic acid, 2,4-dichlorophenoxyacetic acid, 2-(4-chloro-2-methylphenoxy)propionic acid, 2-(2,4-dichlorophenoxy)propionic acid) individually and in a ternary mixture were examined with the comet assay. Data showed that among the active ingredients only 2,4-D andMCPA induced DNA damage, while both herbicides were genotoxic at high concentrations.
Journal of Toxicology and Environmental Health. Part A: Current Issues, 2013, Vol 76, Issue 20