Pyrosequencing represents one of the most thorough methods used to analyze polymorphisms. One advantage of using pyrosequencing for genotyping is the ability to identify not only single-nucleotide polymorphisms (SNPs) but also tri-allelic variations, insertions and deletions (InDels). In contrast to most other genotyping assays the sequence surrounding the polymorphism provides an internal control making this method highly reliable.
Methods in Molecular Biology (clifton, N.j.), 2014, Vol 1100, p. 123-30
Journal Article; Genotyping Techniques; Humans; Lectins; Mannose-Binding Lectin; Polymerase Chain Reaction; Polymorphism, Single Nucleotide; Sequence Analysis, DNA