Kvisgaard, Lise Kirstine1; Hjulsager, Charlotte Kristiane1; Brar, Manreetpal Singh4; Leung, Frederick C. C.4; Larsen, Lars Erik1
1 National Veterinary Institute, Technical University of Denmark2 Section for Virology, National Veterinary Institute, Technical University of Denmark3 Section for Public sector service and commercial diagnostics, National Veterinary Institute, Technical University of Denmark4 University of Hong Kong
Type 2 Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) was first detected in Europe in 1996 co-incident with the introduction of a live attenuated vaccine. Since then, only limited ORF5 and ORF7 sequences of Type 2 PRRS viruses have been reported throughout Europe. In the present study, the genetic and antigenic diversity of 11 complete genomes and 49 ORF5 and 55 ORF7 nucleotide sequences obtained from 57 viruses in Denmark from 2003 to 2012 were examined. The genetic identity of the 11 complete genomes to the vaccine strain (Ingelvac PRRS MLV) ranged between 93.6 and 99.6% while the 49 ORF5 sequences examined were 94.0–99.8% identical to the vaccine strain. Among the Danish sequences, the pairwise nucleotide identity was 90.9–100% and 93.0–100.0% for ORF5 and ORF7, respectively. Analysis of the genetic region encoding NSP2 revealed high diversity among the Danish viruses with an 86.6–98.9% range in similarity. Furthermore, several of the sequenced viruses harbored deletions in the NSP2 coding region. Phylogenetic analysis in a global Type 2 PRRSV framework classified all Danish isolates to a single cluster (sub-lineage 5.1) which comprised strains closely-related to the Type 2 prototype isolate VR2332.
Veterinary Microbiology, 2013, Vol 167, Issue 3-4, p. 334-344
Porcine Reproductive and Respiratory Syndrome Virus (PRRSV); Genotype 2/Type 2; Full genome/complete genome; Non-structural protein 2 (nsp2); Amino acid variations ORF5