Mandrup, Karen2; Jacobsen, Pernille Rosenskjold1; Isling, Louise Krag1; Petersen, Marta Axelstad1; Sørensen, Karin Dreisig1; Hadrup, Niels1; Vinggaard, Anne Marie3; Hass, Ulla1; Boberg, Julie1
1 National Food Institute, Technical University of Denmark2 Division of Toxicology and Risk Assessment, National Food Institute, Technical University of Denmark3 Copenhagen Center for Health Technology, Center, Technical University of Denmark
Perinatal exposure to endocrine disrupting chemicals with estrogenic activity can adversely affect reproductive development, but few studies evaluating estrogen-sensitive endpoints have been performed in Wistar rats. Therefore, time-mated Wistar rats (n=10) were gavaged during gestation and lactation with 0, 5, 15 or 50μg/kg bw/day of ethinyl estradiol.This potent estrogen was found to induce an increased number of nipples and reduced ovary weight in female offspring. Malformations of female genitalia were found in young as well as adult offspring, as an increased AGD was seen at birth and a deeper urethral slit length was seen in adulthood. In prepubertal male offspring, estrogen-regulated gene expression in ventral prostate was increased dose-dependently and a decreased ventral prostate weight was seen at 15μg/kg.Female external sexual characteristics and prostate development were found to be targets for exposure to estrogenic compounds and may be of interest in studies on estrogenic environmental compounds.