Nielsen, Martin Krarup3; Betancourt, Alejandra3; Lyons, Eugene T.3; Horohov, David W.3; Jacobsen, Stine4
1 Section for Medicine and Surgery, Department of Large Animal Sciences, Faculty of Health and Medical Sciences, Københavns Universitet2 University of Kentucky3 University of Kentucky4 Section for Medicine and Surgery, Department of Large Animal Sciences, Faculty of Health and Medical Sciences, Københavns Universitet
Cyathostomins can cause a severe inﬂammation of equine large intestine characterized by substantial ventral edema and pronounced protein loss. Anthelmintic treatment of horses can result in a localized inﬂammatory response in the colonic mucosa of clinically normal horses. The aim of this study was to evaluate the systemic inﬂammatory response of ponies naturally infected with cyathostomins to single dose representatives of three anthelmintic drug classes, namely, oxibendazole, pyrantel pamoate, and moxidectin. Thirty ponies aged between 1 and 18 years of age were allocated to one of three anthelmintic treatments groups. Anthelmintic efﬁcacy was evaluated using the fecal egg count reduction test performed weekly between 2 and 8 weeks post-treatment. Inﬂammatory responses were evaluated on days 0, 1, 3, 5, and 14 after treatment using hematology, measurement of the acute phase inﬂammatory markers serum amyloid A, ﬁbrinogen, haptoglobin, and iron, and real-time PCR measurement of expression of the genes for interleukins 1-b and 10, tumor necrosis factor-a, and interferon-c. There were subtle inﬂammatory responses to treatment, but cytokine expression was signiﬁcantly associated with the interaction term between treatment group and anthelmintic efﬁcacy (P < 0.05). Of the acute phase markers, only ﬁbrinogen associated with treatment group. The ﬁndings suggest that systemic inﬂammatory responses subsequent to anthelmintic treatment of cyathostomin infection are minimal. It is possible that this response is ‘buffered’ by anti-inﬂammatory products of the parasites and/or the anti-inﬂammatory effects of the macrocyclic lactones.
Veterinary Journal, 2013, Vol 198, Issue 2, p. 457-462
The Faculty of Health Science; Horse; Parasite; Veterinary medicine; Inflammation; Acute phase proteins; Serum amyloid A; Cytokines