Thoren, Lina A6; Fog, Cathrine K5; Jensen, Klaus T7; Buza-Vidas, Natalija5; Côme, Christophe7; Lund, Anders H.7; Porse, Bo T6
1 Porse Group, BRIC Research Groups, BRIC, Københavns Universitet2 The Danish Stem Cell Center, Faculty of Health and Medical Sciences, Københavns Universitet3 Lund Group, BRIC Research Groups, BRIC, Københavns Universitet4 Section of Surgery and Internal Medicine, Department of Clinical Medicine, Faculty of Health and Medical Sciences, Københavns Universitet5 unknown6 Porse Group, BRIC Research Groups, BRIC, Københavns Universitet7 Lund Group, BRIC Research Groups, BRIC, Københavns Universitet
Hematopoietic stem cells (HSC)(1) supply organisms with life-long output of mature blood cells. To do so, the HSC pool size has to be maintained by HSC self-renewing divisions. PRDM3 and PRDM16 have been documented to regulate HSC self-renewal, maintenance and function. We found Prdm11 to have similar expression patterns in the hematopoietic stem and progenitor cell (HSPC) compartments as Prdm3 and Prdm16. Therefore, we undertook experiments to test if PRDM11 regulates HSC self-renewal, maintenance and function by investigating the Prdm11(-/-) mice. Our data shows that phenotypic HSPCs are intact in bone marrow (BM) of one-year-old Prdm11(-/-) mice. In addition, Prdm11(-/-) mice were able to fully regenerate the hematopoietic system upon BM transplantation (BMT) into lethally irradiated mice with a mild drop in lymphoid output only. Taken together, this suggests that PRDM11, in contrast to PRDM3 and PRDM16, is not directly involved in regulation of HSPCs in mice.
Stem Cell Research, 2013, Vol 11, Issue 3, p. 1129-1136