Rizzi, Giovanni1; Østerberg, Frederik Westergaard1; Dufva, Martin4; Hansen, Mikkel Fougt1
1 Department of Micro- and Nanotechnology, Technical University of Denmark2 Magnetic Systems, Department of Micro- and Nanotechnology, Technical University of Denmark3 Fluidic Array Systems and Technology, Department of Micro- and Nanotechnology, Technical University of Denmark4 Center for Intelligent Drug Delivery and Sensing Using Microcontainers and Nanomechanics, Center, Technical University of Denmark
We demonstrate a magnetoresistive sensor platform that allows for the real-time detection of point mutations in DNA targets. Specifically, we detect point mutations at two sites in the human beta globin gene. For DNA detection, the present sensor technology has a detection limit of about 160pM and a dynamic range of about two orders of magnitude. The sensors are based on a new geometry for biological sensing that detects the difference between the amount of beads bound to a sensing pad and a local integrated negative reference pad. The magnetic beads are magnetised by the magnetic field arising from the sensor bias current such that no external magnetic fields are needed. The sensors are integrated in a microfluidic system with temperature control. The local negative reference integrated in the sensor geometry efficiently compensates for sensor offsets, external magnetic fields and a uniform background of magnetic beads, which enables real-time quantification of the specific binding of magnetic beads to the sensor surface under varying experimental conditions.
Biosensors and Bioelectronics, 2014, Vol 52, p. 445-451
Biosensor; Magnetoresistive sensor; Single nucleotide polymorphism; Microfluidics; DNA genotyping