1 National Food Institute, Technical University of Denmark2 Division of Food Microbiology, National Food Institute, Technical University of Denmark3 Division of Food Chemistry, National Food Institute, Technical University of Denmark4 University of Auckland5 Department of Systems Biology, Technical University of Denmark6 University of Copenhagen7 Copenhagen Center for Health Technology, Center, Technical University of Denmark
Every single species of the gut microbiota produce low-molecular-weight compounds that are absorbed constantly from the intestinal lumen and carried to systemic circulation where they play a direct role in health and disease. However, very few studies address the host metabolome as a function of colonizing bacteria. In this study the effect of the Lactobacillus acidophilus NCFM strain was investigated by comparing the metabolome of mono-colonized and germ-free mice in several compartments. By liquid-chromatography coupled to mass spectrometry, we were able to show that the metabolome differed between the mono-colonized and germ-free mice, not only in ileum, caecum and colon, but also in plasma and liver. These observations suggest that L. acidophilus NCFM highly influence the metabolism in multiple compartments, underlying that the gut microbiota metabolism affects the host systemic metabolism.
Main Research Area:
Copenhagen Bioscience Conference – Genomics in metabolism, 2013