Lund, Anne Mathilde1; Kildegaard, Helene Faustrup3; Hansen, Bjarne Gram1; Andersen, Mikael Rørdam4; Mortensen, Uffe Hasbro5
1 Department of Systems Biology, Technical University of Denmark2 Center for Microbial Biotechnology, Department of Systems Biology, Technical University of Denmark3 Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark4 Network Engineering of Eukaryotic Cell factories, Department of Biotechnology and Biomedicine, Technical University of Denmark5 Eukaryotic Molecular Cell Biology, Department of Biotechnology and Biomedicine, Technical University of Denmark
The development of the field of mammalian cell factories requests fast and high-throughput methods which means high need for simpler and more efficient cloning techniques. This project applies the ligation-free USERTM (uracil-specific excision reagent) cloning technique to construct mammalian expression vectors with maximum flexibility.
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6th Danish Conference on Biotechnology and Molecular Biology, 2011