1 Department of Systems Biology, Technical University of Denmark2 Agricultural and Environmental Proteomics, Department of Systems Biology, Technical University of Denmark3 Department of Micro- and Nanotechnology, Technical University of Denmark4 Bioanalytics, Department of Micro- and Nanotechnology, Technical University of Denmark5 Enzyme and Protein Chemistry, Department of Systems Biology, Technical University of Denmark6 Fluidic Array Systems and Technology, Department of Micro- and Nanotechnology, Technical University of Denmark7 Center for Intelligent Drug Delivery and Sensing Using Microcontainers and Nanomechanics, Center, Technical University of Denmark8 Enzyme and Protein Chemistry, Department of Biotechnology and Biomedicine, Technical University of Denmark
Programmed cell death (PCD) is a highly regulated process in which cells are dismantled. Reactive oxygen species (ROS) are involved in PCD in plants, but the relationship between and mechanisms behind ROS and PCD are only poorly understood in plant cells compared to in animal cells (Gechev, Tsanko, et al., (2006), BioEssays, 28, p. 1091). Microfluidic cell culture enables in vitro experiments to approach in vivo conditions. Combining microfluidics with the Lab-On-a-Chip concept allows implementing a wide range of assays for real-time monitoring of effects in a biological system of factors such as concentration of selected compounds, external pH, oxygen consumption, redox state and cell viability. The aleurone layer of the barley seed is a 2-3 single cell type thick tissue that can be dissected from the embryo and starchy endosperm. During incubation in vitro this mechanically very robust maintains highly specific responses to the phytohormones gibberellic acid and abscisic acid. Combined with the increasing usage as a model for studying plant protein secretion, these properties make the aleurone layer ideal for maintenance in a microfluidics system (Fath, Angelika, et al., (2001), Plant Physiol, 126, p. 156; Finnie, Christine, et al., (2011), Proteomics, 11, p. 1595). The potential of microfluidics real-time monitoring is relatively unexplored within plant biology, and the barley aleurone layer system will thus enable new ground to be broken in the field of plant science and microfluidics.
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Symposium for Biotech Research at DTU - Systems Biology, 2013