Test samples of two different “advanced” diesel fuels were prepared from the raw diesel of North Sea Light Gas Oil. A commercial “Ultralight” diesel fuel was used as a reference. The three fuels were tested on two engines with and without an oxidation catalyst: 1) a VOLVO 10.0 1 DI was mounted in an engine test bench and a full ECE R49 13 mode test was performed and 2) a VW GOLF 1.6 l engine was mounted in a car and a full transient FTP-75 test was performed. Regulated emissions and unregulated emissions as SOF, sulphur, nitrate, PAH in PM plus vapour phase were measured. Genotoxic activity of the organic solvent extraction fraction from PM and PUF (vapour phase) was determined in a bacterial mutagenesis assay using Salmonella Thyphi-murium TA 98 with and without metabolic activation S9 and TA 98NR.