Bu, Minqiang4; R. Perch-Nielsen, Ivan4; Skov, Julia4; Sørensen, Karen Skotte1; Yi, Sun1; E. Pedersen, Michael4; Hansen, Mikkel Fougt1; W. Harlow, Kenneth4; Wolff, Anders1
1 Department of Micro- and Nanotechnology, Technical University of Denmark2 BioLabChip, Department of Micro- and Nanotechnology, Technical University of Denmark3 Magnetic Systems, Department of Micro- and Nanotechnology, Technical University of Denmark4 DELTA
This paper reports the design, fabrication and experimental verification of a polymer microfluidic labon-a-slide for rapid detection of methicillin-resistant Staphylococcus aureus (MRSA). MRSA cells were captured in a lysis chamber using magnetic beads, followed by thermal lysis. The released DNA was transferred into a second chamber for polymerase chain reaction (PCR) amplification. Fluidic control in the device was accomplished by pneumatic actuation of a micropump and five microvalves integrated on the device. The mecA gene from MRSA was successfully amplified by real-time PCR within 35 min. Presence of the correct PCR amplicon (186 bp) was verified using capillary electrophoresis. The results demonstrate the utility of a low-cost disposable polymer microfluidic system for rapid pathogen detection for point-of-care diagnostics.
Proceedings of the the 17th International Conference on Solid-state Sensors, Actuators and Microsystems, 2013