Sørensen, Mette Aamand4; Jacobsen, Stine5; Petersen, Lars Jelstrup3
1 Section for Medicine and Surgery, Department of Large Animal Sciences, Faculty of Health and Medical Sciences, Københavns Universitet2 Large Animal Medicine & Surgery, Department of Large Animal Sciences, Faculty of Life Sciences, Københavns Universitet3 Institut for Klinisk Medicin - Klinisk fysiologisk afd., Viborg4 Large Animal Medicine & Surgery, Department of Large Animal Sciences, Faculty of Life Sciences, Københavns Universitet5 Section for Medicine and Surgery, Department of Large Animal Sciences, Faculty of Health and Medical Sciences, Københavns Universitet
a review of clinical and experimental findings
Microdialysis is a method for sampling compounds from extracellular ﬂuid with minimal tissue trauma. Small hollow probes that are 0.2–0.5 mm in diameter are inserted into the tissue and slowly perfused. The probe membrane is semi-permeable and a ﬂux of the solutes occurs exclusively according to the concentration gradients. The recovered dialysate reﬂects changes in the composition of the extracellular water phase with a minor time delay. Because microdialysis is a continuous sampling method, it differs from point sample methods, such as blood sampling. The ability to obtain local measurements in the tissues has led to important discoveries in the detection of tissue changes within the areas of pharmacokinetics, pharmacodynamics, pathology and pathophysiology. New technological solutions, such as transportable pumps, ﬂuid collectors and bedside analysers, have made microdialysis an indispensable tool for the surveillance of critically ill human patients, such as after brain injuries and reconstructive surgeries. The use of microdialysis in equine medicine has been sparingly described with only 14 published studies within muscle, pulmonary and hoof lamellar tissue, nasal mucosa, intestinal wall, uterine, allantoic and cerebrospinal ﬂuid and blood. Only a few papers have been published within each area, indicating that few equine researchers are aware of the unique opportunities provided by the technique. This review discusses the theory and applications of microdialysis with a special emphasis on clinical and experimental equine studies, which may be useful to veterinary experimental and clinical researchers.
Journal review article
Veterinary Journal, 2013, Vol 197, Issue 3, p. 553-559
The Faculty of Health and Medical Sciences; Horses; equine; Microdialysis; Veterinary medicine