Andrade Santacoloma, Paloma de Gracia1; Roman Martinez, Alicia1; Sin, Gürkan3; Gernaey, Krist1; Woodley, John1
1 Department of Chemical and Biochemical Engineering, Technical University of Denmark2 Computer Aided Process Engineering Center, Department of Chemical and Biochemical Engineering, Technical University of Denmark3 Centre for oil and gas – DTU, Center, Technical University of Denmark
This paper presents a methodological approach for modeling multi-enzyme in-pot processes. The methodology is exemplified stepwise through the bi-enzymatic production of N-acetyl-D-neuraminic acid (Neu5Ac) from N-acetyl-D-glucosamine (GlcNAc). In this case study, sensitivity analysis is also used to evaluate the reliability of all parameters of the model suggested in literature , Results, from the sensitivity analysis, are used as criteria for a systematic simplification of the model structure. Consequently, model complexity was reduced without compromising the general predictive performance. A deviation of less than 5% from the original model was found.
Computer-aided Chemical Engineering, 2011, p. 1346-1350
Multi-enzyme in-pot; Neu5Ac production; Mathematical model
Main Research Area:
Computer - Aided Chemical Engineering
21st European Symposium on Computer Aided Process Engineering, 2011