Shabala, Sergey7; Bækgaard, Lone3; Shabala, Lana7; Cuin, Tracey A.4; Nemchinov, Lev G.5; Palmgren, Michael Broberg8
1 Transport Biology, Department of Plant Biology, Faculty of Life Sciences, Københavns Universitet2 University of Tasmania3 Novozymes A/S4 INRA-SupAgro5 Agricultural Research Service6 Section for Transport Biology, Department of Plant and Environmental Sciences, Faculty of Science, Københavns Universitet7 University of Tasmania8 Section for Transport Biology, Department of Plant and Environmental Sciences, Faculty of Science, Københavns Universitet
Although the role of Ca2+ influx channels in oxidative stress signaling and cross-tolerance in plants is well established, little is known about the role of active Ca2+ efflux systems in this process. In our recent paper,17 we reported Potato Virus X (PVX)-induced acquired resistance to oxidative stress in Nicotiana benthamiana and showed the critical role of plasma membrane Ca2+/H+ exchangers in this process. The current study continues this research. Using biochemical and electrophysiological approaches, we reveal that both endomembrane P2A and P2B Ca2+-ATPases play significant roles in adaptive responses to oxidative stress by removing excessive Ca2+ from the cytosol, and that their functional expression is significantly altered in PVX-inoculated plants. These findings highlight the crucial role of Ca2+ efflux systems in acquired tolerance to oxidative stress and open up prospects for practical applications in agriculture, after in-depth comprehension of the fundamental mechanisms involved in common responses to environmental factors at the genomic, cellular and organismal levels.
Plant Signalling and Behavior, 2011, Vol 6, Issue 7, p. 1053-1056