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Next-generation sequencing

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Authors:
  • Rieneck, Klaus ;
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    Department of Clinical Medicine, Faculty of Health and Medical Sciences, Københavns Universitet
  • Bak, Mads ;
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    Orcid logo0000-0003-2762-1002
    Medical Genetics Program, Department of Cellular and Molecular Medicine, Faculty of Health and Medical Sciences, Københavns Universitet
  • Jønson, Lars ;
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    Teaching, Department of Biomedical Sciences, Faculty of Health and Medical Sciences, Københavns Universitet
  • Clausen, Frederik Banch ;
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    unknown
  • Krog, Grethe Risum ;
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    unknown
  • Tommerup, Niels ;
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    Medical Genetics Program, Department of Cellular and Molecular Medicine, Faculty of Health and Medical Sciences, Københavns Universitet
  • Nielsen, Leif Kofoed ;
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    Department of Clinical Medicine, Faculty of Health and Medical Sciences, Københavns Universitet
  • Hedegaard, Morten ;
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    Department of Clinical Medicine, Faculty of Health and Medical Sciences, Københavns Universitet
  • Dziegiel, Morten Hanefeld
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    Orcid logo0000-0001-8034-1523
    Section of Diagnostic Sciences, Department of Clinical Medicine, Faculty of Health and Medical Sciences, Københavns Universitet
Subtitle:
proof of concept for antenatal prediction of the fetal Kell blood group phenotype from cell-free fetal DNA in maternal plasma
DOI:
10.1111/trf.12172
Abstract:
BACKGROUND: Maternal immunization against KEL1 of the Kell blood group system can have serious adverse consequences for the fetus as well as the newborn baby. Therefore, it is important to determine the phenotype of the fetus to predict whether it is at risk. We present data that show the feasibility of predicting the fetal KEL1 phenotype using next-generation sequencing (NGS) technology. STUDY DESIGN AND METHODS: The KEL1/2 single-nucleotide polymorphism was polymerase chain reaction (PCR) amplified with one adjoining base, and the PCR product was sequenced using a genome analyzer (GAIIx, Illumina); several millions of PCR sequences were analyzed. RESULTS: The results demonstrated the feasibility of diagnosing the fetal KEL1 or KEL2 blood group from cell-free DNA purified from maternal plasma. CONCLUSION: This method requires only one primer pair, and the large amount of sequence information obtained allows well for statistical analysis of the data. This general approach can be integrated into current laboratory practice and has numerous applications. Besides DNA-based predictions of blood group phenotypes, platelet phenotypes, or sickle cell anemia, and the determination of zygosity, various conditions of chimerism could also be examined using this approach. To our knowledge, this is the first report focused on antenatal blood group determination using NGS.
Type:
Journal article
Language:
English
Published in:
Transfusion, 2013, Vol 53, Issue 11 Suppl 2, p. 2892-2898
Keywords:
Managers and employees at University colleges; Managers and employees at universities, research institutions etc.; Students at University colleges; University students; DNA; Female; Fetal Blood; High-Throughput Nucleotide Sequencing; Humans; Infant, Newborn; Kell Blood-Group System; Maternal-Fetal Exchange; Mothers; Phenotype; Pregnancy; Prenatal Diagnosis; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't
Main Research Area:
Medical science
Publication Status:
Published
Review type:
Peer Review
Submission year:
2013
Scientific Level:
Scientific
ID:
247282572

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