Structural and dynamical aspects of skin studied by multiphoton excitation fluorescence microscopy-based methods
- Authors:
-
-
Bloksgaard, Maria
;
Close
0000-0002-4474-9553
Kardiovaskulær og Renal Forskning, Department of Molecular Medicine, Faculty of Health Sciences, SDU - Brewer, Jonathan R. ;
- Bagatolli, Luis
-
Bloksgaard, Maria
;
- DOI:
- 10.1016/j.ejps.2013.04.010
- Abstract:
- This mini-review reports on applications of particular multiphoton excitation microscopy-based methodologies employed in our laboratory to study skin. These approaches allow in-depth optical sectioning of the tissue, providing spatially resolved information on specific fluorescence probes' parameters. Specifically, by applying these methods, spatially resolved maps of water dipolar relaxation (generalized polarization function using the 6-lauroyl-2-(N,N-dimethylamino)naphthale probe), activity of protons (fluorescence lifetime imaging using a proton sensitive fluorescence probe--2,7-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein) and diffusion coefficients of distinct fluorescence probes (raster imaging correlation spectroscopy) can be obtained from different regions of the tissue. Comparative studies of different tissue strata, but also between equivalent regions of normal and abnormal excised skin, including applications of fluctuation correlation spectroscopy on transdermal penetration of liposomes are presented and discussed. The data from the different studies reported reveal the intrinsic heterogeneity of skin and also prove these strategies to be powerful noninvasive tools to explore structural and dynamical aspects of the tissue.
- Type:
- Journal article
- Language:
- English
- Published in:
- European Journal of Pharmaceutical Sciences, 2013, Vol 50, Issue 5, p. 586-594
- Main Research Area:
- Science/technology
- Publication Status:
- Published
- Review type:
- Peer Review
- Submission year:
- 2013
- Scientific Level:
- Scientific
- ID:
- 247164836
