Støy, Ann Cathrine Findal1; Heegaard, Peter M. H.1; Thymann, Thomas4; Bjerre, Mette5; Skovgaard, Kerstin1; Boye, Mette6; Stoll, Barbara8; Schmidt, Mette4; Jensen, Bent B.5; Sangild, Per Torp4
1 National Veterinary Institute, Technical University of Denmark2 Section for Immunology and Vaccinology, National Veterinary Institute, Technical University of Denmark3 Section for Bacteriology, Pathology and Parasitology, National Veterinary Institute, Technical University of Denmark4 University of Copenhagen5 Aarhus University6 Department of Microbiology, Technical University of Denmark7 Baylor College of Medicine8 Baylor College of Medicine
Background & aims Only few hours of formula feeding may induce proinflammatory responses and predispose to necrotizing enterocolitis (NEC) in preterm pigs. We hypothesized that bovine colostrum, rich in bioactive factors, would improve intestinal function in preterm pigs following an initial exposure to formula feeding after some days of total parenteral nutrition (TPN). Methods After receiving TPN for 2 days, preterm pigs were fed formula (FORM, n = 14), bovine colostrum (COLOS, n = 6), or formula (6 h) followed by bovine colostrum (FCOLOS, n = 14). Intestinal lesions, function, and structure, abundance and location of bacteria, and inflammation markers were investigated. Results NEC severity and interleukins (IL)-1β and -8 protein concentrations were lower, while villus height, galactose absorption, and brush-border enzyme activities were increased in the distal small intestine in COLOS and FCOLOS pigs, relative to FORM pigs. Intestinal gene expression of serum amyloid A, IL-1β, -6 and -8, and bacterial abundance, correlated positively with NEC severity of the distal small intestine. Conclusions Bovine colostrum restores intestinal function after initial formula-induced inflammation in preterm pigs. Further studies are required to test if bovine colostrum may also benefit preterm infants during the challenging transition from total parenteral nutrition to enteral nutrition, when human milk is unavailable.
Clinical Nutrition, 2014, Vol 33, Issue 2, p. 322-329
Bovine colostrum; Necrotizing enterocolitis; Inflammation; Animal model