1 Department of Chemistry, Technical University of Denmark2 Organic Chemistry, Department of Chemistry, Technical University of Denmark3 Center for Hyperpolarization in Magnetic Resonance, Center, Technical University of Denmark
Uptake and upshot in vivo: Straightforward methods that permit the real-time observation of organic acid influx, intracellular acidification, and concomitant effects on cellular-reaction networks are crucial for improved bioprocess monitoring and control (see scheme). Herein, dynamic nuclear polarization (DNP) NMR is used to observe acetate influx, ensuing intracellular acidification and the metabolic consequences on alcoholic fermentation and glycolysis in living cells.
Chemistry: a European Journal, 2013, Vol 19
Acetate; Bioethanol; In vivo spectroscopy; NMR spectroscopy; Yeast fermentation