Vendel Nielsen, Lone7; Hansen, Toke Peter Krogager2; Young, Clifford5; Ferreri, Carla6; Chatgilialoglu, Chryssostomos6; Jensen, Ole Nørregaard5; Enghild, Jan Johannes7
1 iNano-School, Science and Technology, Aarhus University2 iNano-School, Faculty of Science, Aarhus University, Aarhus University3 Department of Molecular Biology and Genetics - Protein science, Department of Molecular Biology and Genetics, Science and Technology, Aarhus University4 Interdisciplinary Nanoscience Center - INANO-MBG, Gustav Wied 10, Interdisciplinary Nanoscience Center, Science and Technology, Aarhus University5 Institut for Biokemi og Molekylær Biologi6 I.S.O.F. — Consiglio Nazionale delle Ricerche7 Department of Molecular Biology and Genetics - Protein science, Department of Molecular Biology and Genetics, Science and Technology, Aarhus University
Trans fatty acid consumption in the human diet can cause adverse health effects, such as cardiovascular disease, which is associated with higher total cholesterol, a higher low density lipoprotein-cholesterol level and a decreased high density lipoprotein-cholesterol level. The aim of the study was to elucidate the hepatic response to the most abundant trans fatty acid in the human diet, elaidic acid, to help explain clinical findings on the relationship between trans fatty acids and cardiovascular disease. The human HepG2 cell line was used as a model to investigate the hepatic response to elaidic acid in a combined proteomic, transcriptomic and lipidomic approach. We found many of the proteins responsible for cholesterol synthesis up-regulated together with several proteins involved in the esterification and hepatic import/export of cholesterol. Furthermore, a profound remodeling of the cellular membrane occurred at the phospholipid level. Our findings contribute to the explanation on how trans fatty acids from the diet can cause modifications in plasma cholesterol levels by inducing abundance changes in several hepatic proteins and the hepatic membrane composition.