Honore, Anders H.4; Thorsen, Michael3; Skov, Thomas5
1 Chemometrics and Analytical Technology, Department of Food Science, Faculty of Science, Københavns Universitet2 Quality & Technology, Department of Food Science, Faculty of Life Sciences, Københavns Universitet3 DuPont Nutrition Biosciences ApS4 Quality & Technology, Department of Food Science, Faculty of Life Sciences, Københavns Universitet5 Chemometrics and Analytical Technology, Department of Food Science, Faculty of Science, Københavns Universitet
Co-cultures of specific lactic and propionic acid bacteria have been shown to have an antagonistic effect against yeast and moulds in dairy systems. In studies of these co-cultures by bioassay-guided fractionation and analysis, numerous compounds have been reported to inhibit yeast and moulds. Although active, the compounds do not account for the full effect observed. Instead, the inhibitory action in the co-culture is believed to be a result of synergy between known exo-metabolites, depletion of nutrients, and/or compounds not yet identified. Untargeted metabolomics or metabolic footprinting could be a potent approach to elucidation of the mechanism. The purpose of this review is to discuss the two pre-requisites for such a study-the compound classes expected in the co-cultures, and on the basis of these, the most suitable analytical technique(s). Ultrahigh-performance liquid chromatography (UPLC) coupled to high-resolution mass spectrometry (MS) via electrospray ionisation (ESI) operated in both positive and negative modes is regarded as the optimum instrumental technique. The applicability of a range of liquid chromatographic techniques ranging from ion-pair (IPC) and hydrophilic interaction (HILIC) to reversed-phase chromatography (RPC) is discussed in terms of the expected metabolome. Use of both HILIC and RPC is suggested, on account of the complementarity of these modes. The most promising strategy uses a combination of the two electrospray polarities and two modes of LC. The strategy recommended in this study does not include all compound classes, and suggestions for supplementary methods are listed.
Analytical and Bioanalytical Chemistry, 2013, Vol 405, Issue 25, p. 8151-8170
Bacterial co-cultures; Exo-metabolome LC-MS; Modes of separation