1 Food Safety and Zoonoses, Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, Københavns Universitet2 Analytical Biosciences, Department of Pharmacy, Faculty of Health and Medical Sciences, Københavns Universitet3 Klima og Bioenergi4 Department of Geochemistry, University of Copenhagen5 Section of Microbiology, Department of Veterinary Disease Biology, Faculty of Life Sciences, Københavns Universitet6 Institut for Agroøkologi - Jordbiologi og Næringsstoffer7 Section of Microbiology, Department of Veterinary Disease Biology, Faculty of Life Sciences, Københavns Universitet8 Analytical Biosciences, Department of Pharmacy, Faculty of Health and Medical Sciences, Københavns Universitet
The redistribution and fate of contaminants in pig slurry after direct injection were investigated at two field sites, Silstrup (sandy clay loam) and Estrup (sandy loam), in Denmark. Intact soil samples were collected for up to seven weeks after slurry injection and concentrations of Salmonella Typhimurium Bacteriophage 28B (phage 28B), Escherichia coli, steroid hormones and other slurry components (water, volatile solids, chloride and mineral N) determined in and around the injection slit. The two experiments at Silstrup and Estrup differed with respect to slurry solid content (6.3 vs. 0.8%), as well as soil clay content (27 vs. 15%) and differed considerably with respect to the initial redistribution of slurry-borne contaminants in soil. The transport of microorganisms from the slurry injection slit to the surrounding soil was much lower than that of mineral N and chloride due to attachment and entrapment. The redistribution of E. coli was more affected by site-specific conditions compared to phage 28B, possibly due to the larger cell size of E. coli. The overall recovery of phage 28B was 0.8-4%, and of E. coli 0.0-1.3% in different samples, by the end of the study. Nine different steroid hormones were detected in the slurry slit, and a slow redistribution to the surrounding soil was observed. Overall recovery of estrogens was 0.0 to 6.6% in different samples. The study showed that the combination of soil and slurry properties determined the initial spreading of contaminants, and hence the potential for subsequent leaching.
Science of the Total Environment, 2014, Vol 466-467, p. 1003-1010
Animals; Colony Count, Microbial; Denmark; Escherichia coli; Gas Chromatography-Mass Spectrometry; Gonadal Steroid Hormones; Manure; Nitrogen; Salmonella Phages; Soil Microbiology; Soil Pollutants; Sus scrofa; Tandem Mass Spectrometry; water treatment; aecal contaminati on; heavy metals; food hygiene; waste- water nutrients value