Dantas, Gautam6; Sommer, Morten1; Degnan, Patrick H.7; Goodman, Andrew L.7
1 Department of Systems Biology, Technical University of Denmark2 Drug Resistance and Community Dynamics, Department of Systems Biology, Technical University of Denmark3 Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark4 Bacterial Cell Factories, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark5 CFB - Core Flow, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark6 Washington University School of Medicine7 Yale University School of Medicine
The complex and intimate relationship between humans and their gut microbial communities is becoming less obscure, due in part to large-scale gut microbial genome-sequencing projects and culture-independent surveys of the composition and gene content of these communities.These studies build upon, and are complemented by, experimental efforts to define underlying mechanisms of host-microbe interactions in simplified model systems. This review highlights the intersection of these approaches. Experimental studies now leverage the advances in high-throughput DNA sequencing that have driven the explosion ofmicrobial genome and community profiling projects, and the loss-of-function and gain-of-function strategies long employed in model organisms are now being extended to microbial genes, species, and communities from the human gut. These developments promise to deepen our understanding of human gut host–microbiota relationships and are readily applicable to other host-associated and free-living microbial communities.
Annual Review of Microbiology, 2013, Vol 67, p. 459-475