1 Department of Biology, Faculty of Science, Københavns Universitet2 Genome Research and Molecular Bio Medicine, Department of Biology, Faculty of Science, Københavns Universitet3 National Institute of Nutrition and Seafood Research4 Aarhus Universitet5 University of Bergen6 Department of Biology, Faculty of Science, Københavns Universitet7 Genome Research and Molecular Bio Medicine, Department of Biology, Faculty of Science, Københavns Universitet8 University of Bergen
The digestion rate of dietary protein is a regulating factor for postprandial metabolism both in humans and animal models. However, few data exist about the habitual consumption of proteins with different digestion rates with regard to the development of body mass and diet-induced obesity. Here, we used a factorial ANOVA design to investigate the effects of protein form (intact vs. hydrolyzed casein) and protein level (16 vs. 32 energy percent protein) on body mass gain and adiposity in obesity-prone male C57BL/6J mice fed Western diets with 35 energy percent fat. Mice fed the hydrolyzed casein diets had higher spontaneous locomotor activity than mice fed intact casein. During the light phase, mice fed hydrolyzed casein tended (P = 0.08) to have a lower respiratory exchange ratio, indicating lower utilization of carbohydrates as energy substrate relative to those fed intact casein. In further support of less carbohydrate oxidation, plasma concentrations of glucose and those of the glucose metabolite lactate were lower in fed mice that consumed the hydrolyzed compared with the intact casein diet. Concomitantly, the plasma insulin concentration was strongly reduced in fed mice given hydrolyzed casein relative to those given intact casein. The mice fed hydrolyzed casein had greater ex vivo inguinal white adipose tissue non-CO2 β-oxidation capacity along with induced expression of genes involved in mitochondrial fatty acid oxidation and mitochondrial uncoupling. The physiological changes induced by hydrolyzed casein ingestion translated into decreased body and adipose tissue masses. We conclude that chronic consumption of extensively hydrolyzed casein reduces body mass gain and diet-induced obesity in male C57BL/6J mice.
Journal of Nutrition, 2013, Vol 143, Issue 9, p. 1367-1375
Adiposity; Animals; Blood Glucose; Body Composition; Body Mass Index; Calorimetry, Indirect; Caseins; Diet; Dietary Proteins; Glucose Tolerance Test; Insulin; Lipid Metabolism; Magnetic Resonance Imaging; Male; Mice; Mice, Inbred C57BL; Obesity; Postprandial Period; Weight Gain