- Authors:
-
-
Abou Hachem, Maher
;
Close
0000-0001-8250-1842
Enzyme and Protein Chemistry, Department of Systems Biology, Technical University of Denmark - Andersen, Joakim Mark ;
- Barrangou, Rodolphe ;
-
Møller, Marie Sofie
;
Close0000-0001-9017-3367
Enzyme and Protein Chemistry, Department of Systems Biology, Technical University of Denmark -
Fredslund, Folmer
;
Close0000-0003-0881-1927
Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark - Majumder, Avishek ;
-
Hansen, Morten Ejby
;
Close0000-0003-2099-3591
Department of Biotechnology and Biomedicine, Technical University of Denmark - Lahtinen, Sampo J. ;
- Jacobsen, Susanne ;
- Lo Leggio, Leila ;
- Goh, Yong Jun ;
- Klaenhammer, Todd R. ;
-
Svensson, Birte
Close0000-0002-2993-8196
Enzyme and Protein Chemistry, Department of Systems Biology, Technical University of Denmark
-
Abou Hachem, Maher
;
- DOI:
- 10.3109/10242422.2013.828048
- Abstract:
- In recent years, a plethora of studies have demonstrated the paramount physiological importance of the gut microbiota on various aspects of human health and development. Particular focus has been set on probiotic members of this community, the best studied of which are assigned into the Lactobacillus and Bifidobacterium genera. Effects such as pathogen exclusion, alleviation of inflammation and allergies, colon cancer, and other bowel disorders are attributed to the activity of probiotic bacteria, which selectively ferment prebiotics comprising mainly non-digestible oligosaccharides. Thus, glycan metabolism is an important attribute of probiotic action and a factor influencing the composition of the gut microbiota. In the quest to understand the molecular mechanism of this selectivity for certain glycans, we have explored the routes of uptake and utilization of a variety of oligosaccharides differing in size, composition, and glycosidic linkages. A combination of "omics" technologies bioinformatics, enzymology and protein characterization proved fruitful in elucidating the protein transport and catabolic machinery conferring the utilization of glucosides, galactosides, and xylosides in the two clinically validated probiotic strains Lactobacillus acidophilus NCFM and Bifidobacterium animalis subsp. lactis Bl-04. Importantly, we have been able to identify and in some cases validate the specificity of several transport systems, which are otherwise poorly annotated. Further, we have demonstrated for the first time that non-naturally occurring tri- and tetra-saccharides are internalized and efficiently utilized by probiotic bacteria in some cases better than well-established natural prebiotics. Selected highlights of these data are presented, emphasising the importance and the diversity of oligosaccharide transport in probiotic bacteria. © 2013 Informa UK, Ltd.
- Type:
- Conference paper
- Language:
- English
- Published in:
- Biocatalysis and Biotransformation, 2013, Vol 31, Issue 4, p. 226-235
- Keywords:
- Bacteria; Bioinformatics; Machinery; Metabolism; Physiology; Polysaccharides; Proteins; Oligosaccharides; Prebiotic; oligosaccharide uptake; ATP-binding cassette transport system; phosphotransferase system; transcriptional analysis
- Main Research Area:
- Science/technology
- Publication Status:
- Published
- Review type:
- Peer Review
- Conference:
- 10th Carbohydrate Bioengineering Meeting, 2013
- Submission year:
- 2013
- Scientific Level:
- Scientific
- ID:
- 244404843
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