Jensen, Michael Krogh3; Lindemose, Søren4; De Masi, Federico5; Reimer, Julia J6; Nielsen, Michael4; Perera, Venura9; Workman, Christopher8; Turck, Franziska6; Grant, Murray R9; Mundy, John4; Petersen, Morten4; Skriver, Karen4
1 Department of Systems Biology, Technical University of Denmark2 Center for Biological Sequence Analysis, Department of Systems Biology, Technical University of Denmark3 Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark4 University of Copenhagen5 Department of Bio and Health Informatics, Technical University of Denmark6 Max Planck Institute7 University of Exeter8 Regulatory Genomics, Department of Biotechnology and Biomedicine, Technical University of Denmark9 University of Exeter
ATAF1, an Arabidopsis thaliana NAC transcription factor, plays important roles in plant adaptation to environmental stress and development. To search for ATAF1 target genes, we used protein binding microarrays and chromatin-immunoprecipitation (ChIP). This identified T[A,C,G]CGT[A,G] and TT[A,C,G]CGT as ATAF1 consensus binding sequences. Co-expression analysis across publicly available microarray experiments identified 25 genes co-expressed with ATAF1. The promoter regions of ATAF1 co-expressors were significantly enriched for ATAF1 binding sites, and TTGCGTA was identified in the promoter of the key abscisic acid (ABA) phytohormone biosynthetic gene NCED3. ChIP-qPCR and expression analysis showed that ATAF1 binding to the NCED3 promoter correlated with increased NCED3 expression and ABA hormone levels. These results indicate that ATAF1 regulates ABA biosynthesis.