Knud J. Jensen, Pernille Tofteng Shelton, Søren L. Pedersen
1 Administration, Department of Chemistry, Faculty of Science, Københavns Universitet2 Novo Nordisk A/S3 Administration, Department of Chemistry, Faculty of Science, Københavns Universitet
O-glycosylation of proteins is an important modification which affects biological function and immunity. In this chapter, we provide protocols for efficient solid-phase O-glycopeptide synthesis (SPGPS) and protocols for the construction of glycopeptide microarray chips for screening applications. This will be exemplified for mucin-type glycopeptides and the construction of glycopeptide microarrays. To this end, the protocols provided are particularly suited for small-scale robotic parallel synthesis. N-Terminal amine capping of deletion peptides during synthesis stands out as vital to this strategy. It allows for direct on-slide enrichment of the full-length target product and thereby bypasses tedious isolation and purification procedures.