Shelton, Anne Pernille Tofteng3; Jensen, Knud Jørgen4
Knud J. Jensen, Pernille T. Shelton, Søren L. Pedersen
1 Administration, Department of Chemistry, Faculty of Science, Københavns Universitet2 Chemistry and Biochemistry, Department of Basic Sciences and Environment, Faculty of Life Sciences, Københavns Universitet3 Chemistry and Biochemistry, Department of Basic Sciences and Environment, Faculty of Life Sciences, Københavns Universitet4 Administration, Department of Chemistry, Faculty of Science, Københavns Universitet
This chapter describes the basic protocols for solid-phase peptide synthesis using the Fmoc group as the N-alpha-protecting group (Fmoc-SPPS). The chapter introduces resins and their handling, choice of linkers, and the most common methods for peptide chain assembly. The proper choice of resins and linkers for solid-phase synthesis is a key parameter for successful peptide synthesis. This chapter provides an overview of the most common and useful resins and linkers for the synthesis of peptides with C-terminal amides, carboxylic acids, and more. The chapter finishes with robust protocols for general solid-phase peptide synthesis, i.e., the standard operations.