1 Department of Systems Biology, Technical University of Denmark2 Natural Product Chemistry, Department of Systems Biology, Technical University of Denmark3 University of California4 Prosolia Inc.5 National Veterinary Institute, Technical University of Denmark6 University of Veterinary and Pharmaceutical Sciences7 San Diego State University8 Harvard University9 Chinese Academy of Sciences10 University of Veterinary and Pharmaceutical Sciences11 San Diego State University12 Harvard University
Microorganisms such as bacteria and fungi produce a variety of specialized metabolites that are invaluable for agriculture, biological research, and drug discovery. However, the screening of microbial metabolic output is usually a time-intensive task. Here, we utilize a liquid microjunction surface sampling probe for electrospray ionization-mass spectrometry to extract and ionize metabolite mixtures directly from living microbial colonies grown on soft nutrient agar in Petri-dishes without any sample pretreatment. To demonstrate the robustness of the method, this technique was applied to observe the metabolic output of more than 30 microorganisms, including yeast, filamentous fungi, pathogens, and marine-derived bacteria, that were collected worldwide. Diverse natural products produced from different microbes, including Streptomyces coelicolor, Bacillus subtilis, and Pseudomonas aeruginosa are further characterized.
Analytical Chemistry, 2013, Vol 85, Issue 15, p. 7014-7018