Vester, Jan Kjølhede3; Glaring, Mikkel Andreas3; Stougaard, Peter4
1 Microbial Ecology and Biotechnology, Department of Plant and Environmental Sciences, Faculty of Science, Københavns Universitet2 Department of Agriculture & Ecology, Genetics & Microbiology, Department of Agriculture & Ecology, Faculty of Life Sciences, Københavns Universitet3 Department of Agriculture & Ecology, Genetics & Microbiology, Department of Agriculture & Ecology, Faculty of Life Sciences, Københavns Universitet4 Microbial Ecology and Biotechnology, Department of Plant and Environmental Sciences, Faculty of Science, Københavns Universitet
The ikaite columns in the Ikka Fjord in Greenland represent one of the few permanently cold and alkaline environments on Earth, and the interior of the columns is home to a bacterial community adapted to these extreme conditions. The community is characterized by low cell numbers imbedded in a calcium carbonate matrix, making extraction of bacterial cells and DNA a challenge and limiting molecular and genomic studies of this environment. To utilize this genetic resource, cultivation at high pH and low temperature was studied as a method for obtaining biomass and DNA from the fraction of this community that would not otherwise be amenable to genetic analyses. The diversity and community dynamics in mixed cultures of bacteria from ikaite columns was investigated using denaturing gradient gel electrophoresis and pyrosequencing of 16S rDNA. Both medium composition and incubation time influenced the diversity of the culture and many hitherto uncharacterized genera could be brought into culture by extended incubation time. Extended incubation time also gave rise to a more diverse community with a significant number of rare species not detected in the initial community.
Canadian Journal of Microbiology, 2013, Vol 59, Issue 8, p. 581-586