Soares, Siomar C2; Trost, Eva3; Ramos, Rommel T J3; Carneiro, Adriana R3; Santos, Anderson R3; Pinto, Anne C3; Barbosa, Eudes Guilherme Vieria5; Aburjaile, Flávia3; Ali, Amjad3; Diniz, Carlos A A3; Hassan, Syed S3; Fiaux, Karina3; Guimarães, Luis C3; Bakhtiar, Syeda Marriam4; Pereira, Ulisses3; Almeida, Sintia S3; Abreu, Vinícius A C3; Rocha, Flávia S3; Dorella, Fernanda A3; Miyoshi, Anderson3; Silva, Artur3; Azevedo, Vasco3; Tauch, Andreas3
1 Department of Mathematics and Computer Science (IMADA), Faculty of Science, SDU2 CLIB Graduate Cluster Industrial Biotechnology, Centrum für Biotechnologie, Universität Bielefeld, 33615 Bielefeld, Germany. email@example.com unknown4 Wilhelm Johannsen Center5 Department of Mathematics and Computer Science (IMADA), Faculty of Science, SDU
Corynebacterium pseudotuberculosis is the causative agent of several veterinary diseases in a broad range of economically important hosts, which can vary from caseous lymphadenitis in sheep and goats (biovar ovis) to ulcerative lymphangitis in cattle and horses (biovar equi). Existing vaccines against C. pseudotuberculosis are mainly intended for small ruminants and, even in these hosts, they still present remarkable limitations. In this study, we present the complete genome sequence of C. pseudotuberculosis biovar equi strain 258, isolated from a horse with ulcerative lymphangitis. The genome has a total size of 2,314,404 bp and contains 2088 predicted protein-coding regions. Using in silico analysis, eleven pathogenicity islands were detected in the genome sequence of C. pseudotuberculosis 258. The application of a reverse vaccinology strategy identified 49 putative antigenic proteins, which can be used as candidate vaccine targets in future works.
Philippine Journal of Biotechnology, 2013, Vol 167, Issue 2, p. 135-141