Transient activation of mTOR following forced treadmill exercise in rats

Betina Elfving; Tina Christensen; Cecilia Friis Ratner; Jacob Wienecke; Anders Bue Klein

doi:10.1002/syn.21668

Transient activation of mTOR following forced treadmill exercise in rats

Authors:

Elfving, Betina ;
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Department of Clinical Medicine - Translational Neuropsychiatry Unit, Department of Clinical Medicine, Health, Aarhus University
Christensen, Tina ;
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Department of Clinical Epidemiology, Faculty of Health Sciences, Aarhus University, Aarhus University
Ratner, Cecilia Friis ;
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Metabolic Receptology
Wienecke, Jacob ;
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Fysisk aktivitet og hjernen
Klein, Anders Bue
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Institut for Klinisk Medicin

DOI:

10.1002/syn.21668

Abstract:

The beneficial effect of exercise on hippocampal plasticity is possibly mediated by increased angiogenesis and neurogenesis. In angiogenesis insulin-like growth factor-1 (IGF-1), vascular endothelial growth factor (VEGF), and hypoxia-inducible factor 1, alpha subunit (HIF1a) are important factors, while the induction of neurogenesis requires signaling through the VEGF receptor, Flk-1 (VEGFR-2). VEGF expression is believed to be regulated by two distinct mTOR (mammalian Target of Rapamycin)-containing multiprotein complexes mTORC1 and mTORC2, respectively. This study was initiated to investigate the effect of exercise on the expression of VEGF, cognate receptors, HIF1a, mTORC1, and mTORC2 in hippocampus and frontal cortex. To this end, we measured messenger RNA (mRNA) levels in rat brain using quantitative real-time polymerase chain reaction (real-time qPCR) after forced treadmill exercise for 1 day, 2 weeks, and 8 weeks. Rats were euthanized either immediately (0 hrs) or 24 hours after last exercise session. Here, we show that exercise affected mRNA levels of VEGF, VEGFR2, and the co-receptor neuropilin 2 (NRP2) when the rats were euthanized immediately, whereas at 24 hours only the expression of mTOR was regulated after a single bout of exercise. In conclusion, the effect of treadmill exercise on the VEGF system is acute rather than chronic and there is a transient activation of mTOR. More studies are needed to understand whether this could be beneficial in the treatment of neuropsychiatric disorders. Synapse, 2013. © 2013 Wiley Periodicals, Inc.

Type:

Journal article

Language:

English

Published in:

Synapse (new York), 2013, Vol 67, Issue 9, p. 620-5

Keywords:

Animals; Exercise Test; Frontal Lobe; Hippocampus; Hypoxia-Inducible Factor 1, alpha Subunit; Male; Multiprotein Complexes; Neuropilin-2; Physical Exertion; Rats; Rats, Wistar; TOR Serine-Threonine Kinases; Transcription, Genetic; Vascular Endothelial Growth Factor A; Vascular Endothelial Growth Factor Receptor-2; Journal Article; Research Support, Non-U.S. Gov't

Main Research Area:

Medical science

Publication Status:

Published

Review type:

Peer Review

Submission year:

2013

Scientific Level:

Scientific

ID:

243597949

Transient activation of mTOR following forced treadmill exercise in rats

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