Transient activation of mTOR following forced treadmill exercise in rats

Betina Elfving; Tina Christensen; Cecilia Ratner; Jacob Wienecke; Anders B Klein

doi:10.1002/syn.21668

Transient activation of mTOR following forced treadmill exercise in rats

Authors:

Elfving, Betina ;
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Center for Psychiatric Research, Aarhus University Hospital, Risskov
Christensen, Tina ;
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Institut for Neuroscience og Farmakologi & Institut for Idræt og Ernæring, Københavns Universitet
Ratner, Cecilia ;
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Section for Metabolic Receptology, Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, Københavns Universitet
Wienecke, Jacob ;
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0000-0001-9786-4689
Neural Control of Movement, Department of Nutrition, Exercise and Sports, Faculty of Science, Københavns Universitet
Klein, Anders B
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0000-0002-0236-3089
Experimental Pharmacology, Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, Københavns Universitet

DOI:

10.1002/syn.21668

Abstract:

The beneficial effect of exercise on hippocampal plasticity is possibly mediated by increased angiogenesis and neurogenesis. In angiogenesis insulin-like growth factor-1 (IGF-1), vascular endothelial growth factor (VEGF), and hypoxia-inducible factor 1, alpha subunit (HIF1a) are important factors, while the induction of neurogenesis requires signaling through the VEGF receptor, Flk-1 (VEGFR-2). VEGF expression is believed to be regulated by two distinct mTOR (mammalian Target of Rapamycin)-containing multiprotein complexes mTORC1 and mTORC2, respectively. This study was initiated to investigate the effect of exercise on the expression of VEGF, cognate receptors, HIF1a, mTORC1, and mTORC2 in hippocampus and frontal cortex. To this end, we measured messenger RNA (mRNA) levels in rat brain using quantitative real-time polymerase chain reaction (real-time qPCR) after forced treadmill exercise for 1 day, 2 weeks, and 8 weeks. Rats were euthanized either immediately (0 hrs) or 24 hours after last exercise session. Here, we show that exercise affected mRNA levels of VEGF, VEGFR2, and the co-receptor neuropilin 2 (NRP2) when the rats were euthanized immediately, whereas at 24 hours only the expression of mTOR was regulated after a single bout of exercise. In conclusion, the effect of treadmill exercise on the VEGF system is acute rather than chronic and there is a transient activation of mTOR. More studies are needed to understand whether this could be beneficial in the treatment of neuropsychiatric disorders. Synapse, 2013. © 2013 Wiley Periodicals, Inc.

Type:

Journal article

Language:

English

Published in:

Synapse (new York), 2013, Vol 67, Issue 9, p. 620-625

Keywords:

Journal Article; Research Support, Non-U.S. Gov't; Animals; Exercise Test; Frontal Lobe; Hippocampus; Hypoxia-Inducible Factor 1, alpha Subunit; Male; Multiprotein Complexes; Neuropilin-2; Physical Exertion; Rats; Rats, Wistar; TOR Serine-Threonine Kinases; Transcription, Genetic; Vascular Endothelial Growth Factor A; Vascular Endothelial Growth Factor Receptor-2

Main Research Area:

Medical science

Publication Status:

Published

Review type:

Peer Review

Submission year:

2013

Scientific Level:

Scientific

ID:

243597949

Transient activation of mTOR following forced treadmill exercise in rats

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