The portal of entrance of either formalin inactivated or live Yersinia ruckeri organism in rainbow trout fish was studied by applying immunohistochemistry and in-situ hybridization. The sequential study involved a specific monoclonal antibody and a specific oligonucleotide probe binding to Yersinia ruckeri. It demonstrated the differential and regional uptake of both formalin inactivated and live bacterial organism in rainbow trout. The uptake dynamics in various organs/tissues demonstrated a site specific propensity between formalin inactivated and live bacteria. The possibility that lateral lines, dorsal fins and the gastro-intestinal tract could act as an active avenue to bacterial entrance was shown by both immunohistochemistry and in situ hybridization. The translocations of systemically absorbed formalin inactivated and live bacteria within different host body compartments were elucidated.
Dafinet Workshop and Ph.d. Course: Book of Abstracts, 2013
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DAFINET Workshop : Diagnosis and Control of Fish Diseases, 2013