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1 Department of Micro- and Nanotechnology, Technical University of Denmark 2 Polymer Microsystems for Medical Diagnostics, Department of Micro- and Nanotechnology, Technical University of Denmark 3 Risø National Laboratory for Sustainable Energy, Technical University of Denmark
Influenza infections are associated with high morbidity and mortality, carry the risk of pandemics, and pose a considerable economic burden worldwide. To improve the management of the illness, it is essential with accurate and fast point-of-care diagnostic tools for use in the field or at the patient's bedside. Conventional diagnostic methods are time consuming, expensive and require specialized laboratory facilities. We present a highly sensitive, highly specific, and low cost platform to test for acute virus infections in less than 15. min, employing influenza A virus (H1N1) as an example of its usability. An all polymer microfluidic system with a functionalized conductive polymer (PEDOT-OH:TsO) microelectrode array was developed and exploited for label free and real time electrochemical detection of intact influenza A virus (H1N1) particles. DNA aptamers with affinity for influenza A virus (H1N1) were linked covalently to the conductive polymer microelectrodes in the microfluidic channel. Based on changes in the impedance when virions were captured by immobilized probes, we could detect clinically relevant concentrations of influenza A virus (H1N1) in saliva. This is a new, stable and very sensitive point-of-care platform for detection and diagnostics of intact virus particles. © 2013 Elsevier B.V.
Biosensors and Bioelectronics, 2013, Vol 49, p. 374-379
Electrochemical impedance spectroscopy; Fluidic devices; Microelectrodes; Polymers; Viruses; Conductive polymer; PEDOT-OH:TsO; Electrochemical impedance Ćspectroscopy; (EIS); DNA aptamer; Influenza A virus (H1N1); AFM; Point-of-care (POC)
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