1 Department of Molecular Biology and Genetics - Genome stability and technology, Department of Molecular Biology and Genetics, Science and Technology, Aarhus University2 unknown3 Epidemiologi, Biostatistik og Biodemografi4 Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health5 The Danish Aging Research Center, Epidemiology Unit, Institute of Public Health, University of Southern Denmark6 Department of Molecular Biology and Genetics - Genome stability and technology, Department of Molecular Biology and Genetics, Science and Technology, Aarhus University
A twin study
Accumulation of DNA damage deriving from exogenous and endogenous sources has significant consequences for cellular survival, and is implicated in aging, cancer, and neurological diseases. Different DNA repair pathways have evolved in order to maintain genomic stability. Genetic and environmental factors are likely to influence DNA repair capacity. In order to gain more insight into the genetic and environmental contribution to the molecular basis of DNA repair, we have performed a human twin study, where we focused on the consequences of some of the most abundant types of DNA damage (single-strand breaks), and some of the most hazardous lesions (DNA double-strand breaks). DNA damage signaling response (Gamma-H2AX signaling), relative amount of endogenous damage, and DNA-strand break repair capacities were studied in peripheral blood mononuclear cells from 198 twins (94 monozygotic and 104 dizygotic). We did not detect genetic effects on the DNA-strand break variables in our study.
Environmental and Molecular Mutagenesis, 2013, Vol 54, Issue 6, p. 414-420