Fredborg, Marlene4; Andersen, Klaus R.5; Jørgensen, Erik5; Droce, Aida1; Olesen, Tom5; Jensen, Bent B.4; Rosenvinge, Flemming S.6; Søndergaard, Teis1
1 Section of Biotechnology, The Faculty of Engineering and Science, Aalborg University, VBN2 Department of Chemistry and Bioscience, The Faculty of Engineering and Science, Aalborg University, VBN3 The Faculty of Engineering and Science, Aalborg University, VBN4 Aarhus Universitet5 Unisensor A/S6 SDU
Rapid antibiotic susceptibility testing is in highly demand in health-care fields as antimicrobial resistant bacterial strains emerge and spread. Here we describe an optical screening system (oCelloScope), which based on time-lapse imaging of 96 bacteria-antibiotic combinations at a time, introduces real-time detection of bacterial growth and antimicrobial susceptibility, with imaging material to support the automatically generated graphs. Automated antibiotic susceptibility tests of a monoculture showed statistically significant antibiotic effect within 6 minutes and within 30 minutes in complex samples from pigs suffering from catheter associated urinary tract infections. The oCelloScope system provides a fast high-throughput screening method to detect bacterial susceptibility that may entail earlier diagnosis and introduction of appropriate targeted therapy and thus combat the threat from multidrug-resistant pathogenic bacteria. The oCelloScope system can be employed for a broad range of applications within bacteriology and may present new vistas as a point-of-care instrument in both clinical and veterinarian settings.
Journal of Clinical Microbiology, 2013, Vol 51, Issue 7, p. 2047-2053