1 Department of Photonics Engineering, Technical University of Denmark2 Optical Microsensors and Micromaterials, Department of Photonics Engineering, Technical University of Denmark3 Petru Poni Institute of Macromolecular Chemistry4 National Institute for Laser, Plasma and Radiation Physics5 National Institute for Physics and Nuclear Engineering ‘Horia Hulubei’6 National Institute for Laser, Plasma and Radiation Physics
Synthesized N,N′-(methacryloyloxyethyl triehtoxy silyl propyl carbamoyl-oxyhexyl)-urea hybrid methacrylate was polymerized by direct laser polymerization using femtosecond laser pulses with the aim of using it for subsequent applications in tissue engineering. The as-obtained scaffolds were modified either by low pressure argon plasma treatment or by covering the structures with two different proteins (lysozyme, fibrinogen). For improved adhesion, the proteins were deposited by matrix assisted pulsed laser evaporation technique. The functionalized structures were tested in mouse fibroblasts culture and the cells morphology, proliferation, and attachment were analyzed.
Applied Surface Science, 2013, Vol 278
Ormosil scaffolds; Direct laser writing; Low pressure plasma treatment; MAPLE