Eijsden, Rudy G. E. van3; Stassen, Catherine9; Daenen, Luk10; Mulders, Sebastiaan E. Van10; Bapat, Prashant Madhusudhan1; Siewers, Verena6; Goossens, Katty V. Y.11; Nielsen, Jens6; Delvaux, Freddy R.10; Hummelen, Paul Van12; Devreese, Bart9; Willaert, Ronnie G.11
1 Department of Systems Biology, Technical University of Denmark2 Center for Microbial Biotechnology, Department of Systems Biology, Technical University of Denmark3 VIB Nucleomics Core4 Ghent University5 KU Leuven6 Chalmers University of Technology7 Vrije Universiteit Brussel8 Dana-Farber Cancer Institute9 Ghent University10 KU Leuven11 Vrije Universiteit Brussel12 Dana-Farber Cancer Institute
Genomics, transcriptomics, proteomics and fluxomics are powerful omics-technologies that play a major role in today’s research. For each of these techniques good sample quality is crucial. Major factors contributing to the quality of a sample is the actual sampling procedure itself and the way the sample is stored directly after sampling. It has already been described that RNAlater can be used to store tissues and cells in a way that the RNA quality and quantity are preserved. In this paper, we demonstrate that quaternary ammonium salts (RNAlater) are also suitable to preserve and store samples from Saccharomyces cerevisiae for later use with the four major omics-technologies. Moreover, it is shown that RNAlater also preserves the cell morphology and the potential to recover growth, permitting microscopic analysis and yeast cell culturing at a later stage.
Biotechnology Letters, 2013, Vol 35, Issue 6, p. 891-900