1 Department of Micro- and Nanotechnology, Technical University of Denmark2 Fluidic Array Systems and Technology, Department of Micro- and Nanotechnology, Technical University of Denmark3 Bioanalytics, Department of Micro- and Nanotechnology, Technical University of Denmark4 ChemLabChip, Department of Micro- and Nanotechnology, Technical University of Denmark5 Bioneer A/S6 Risø National Laboratory for Sustainable Energy, Technical University of Denmark7 Copenhagen University Hospital8 Center for Intelligent Drug Delivery and Sensing Using Microcontainers and Nanomechanics, Center, Technical University of Denmark
a holistic approach to microfluidic system design
A microfluidic component library for building systems driving parallel or serial microfluidic-based assays is presented. The components are a miniaturized eight-channel peristaltic pump, an eight-channel valve, sample-to-waste liquid management, and interconnections. The library of components was tested by constructing various systems supporting perfusion cell culture, automated DNA hybridizations, and in situ hybridizations. The results showed that the MainSTREAM components provided (1) a rapid, robust, and simple method to establish numerous fluidic inputs and outputs to various types of reaction chips; (2) highly parallel pumping and routing/valving capability; (3) methods to interface pumps and chip-to-liquid management systems; (4) means to construct a portable system; (5) reconfigurability/flexibility in system design; (6) means to interface to microscopes; and (7) compatibility with tested biological methods. It was found that LEGO Mindstorms motors, controllers, and software were robust, inexpensive, and an accessible choice as compared with corresponding custom-made actuators. MainSTREAM systems could operate continuously for weeks without leaks, contamination, or system failures. In conclusion, the MainSTREAM components described here meet many of the demands on components for constructing and using microfluidics systems.
Journal of Laboratory Automation, 2013, Vol 18, Issue 3, p. 212-228
Microfluidic; Platform; Modular; Pumping; Valving; Multiplexing; Automation; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't; Animals; Automation, Laboratory; Cell Culture Techniques; Cost-Benefit Analysis; HeLa Cells; Holistic Health; Humans; In Situ Hybridization; Microfluidic Analytical Techniques; Miniaturization; Point-of-Care Systems; Reproducibility of Results