Steentoft, Catharina5; Vakhrushev, Sergey5; Joshi, Hiren Jitendra5; Kong, Yun5; Vester-Christensen, Malene B5; Schjoldager, Katrine T-BG5; Lavrsen, Kirstine5; Dabelsteen, Sally Anne Malene5; Pedersen, Nis Borbye5; Marcos-Silva, Lara5; Gupta, Ramneek1; Paul Bennett, Eric5; Mandel, Ulla5; Brunak, Søren1; Wandall, Hans H.5; Levery, Steven Bruce5; Clausen, Henrik5
1 Department of Systems Biology, Technical University of Denmark2 Center for Biological Sequence Analysis, Department of Systems Biology, Technical University of Denmark3 Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark4 CFB - Metagenomic Systems Biology, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark5 University of Copenhagen
Glycosylation is the most abundant and diverse posttranslational modification of proteins. While several types of glycosylation can be predicted by the protein sequence context, and substantial knowledge of these glycoproteomes is available, our knowledge of the GalNAc-type O-glycosylation is highly limited. This type of glycosylation is unique in being regulated by 20 polypeptide GalNAc-transferases attaching the initiating GalNAc monosaccharides to Ser and Thr (and likely some Tyr) residues. We have developed a genetic engineering approach using human cell lines to simplify O-glycosylation (SimpleCells) that enables proteome-wide discovery of O-glycan sites using 'bottom-up' ETD-based mass spectrometric analysis. We implemented this on 12 human cell lines from different organs, and present a first map of the human O-glycoproteome with almost 3000 glycosites in over 600 O-glycoproteins as well as an improved NetOGlyc4.0 model for prediction of O-glycosylation. The finding of unique subsets of O-glycoproteins in each cell line provides evidence that the O-glycoproteome is differentially regulated and dynamic. The greatly expanded view of the O-glycoproteome should facilitate the exploration of how site-specific O-glycosylation regulates protein function.
Embo Journal, 2013, Vol 32, Issue 10, p. 1478-1488